Lentivirus Transduction
Lentivirus Transduction - Related Videos
14:03
Transduction of Human Cells with Polymer-complexed Ecotropic Lentivirus for Enhanced Biosafety
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Cited by 8 •
2011
Lentiviruses are a valuable research tool for exploring gene function; however, researchers may wish to avoid production of pantropic lentivirus encoding known or suspected oncogenes. As an alternative, we present a safer protocol for use of ecotropic lentivirus on human cells modified to express the ecotropic receptor mSlc7a1.
03:19
Transduction to Label PDX Tumor Cells: Introducing Lentivirus Expressing Fluorescent Marker into the Tumor Cell In Vitro
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2023
The following video describes a technique of transduction to label (patient-derived xenografts) PDX tumor cells, which is used for introducing lentivirus expressing green-fluorescent protein and luciferase reporters into the tumor cell in vitro.
11:42
Lentivirus Production
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Cited by 43 •
2009
To make lentiviruses, DNA vectors are transfected into human 293 cells. After harvest and concentrating the supernatant, virus titer is determined by fluorescence expression with a flow cytometer.
11:34
Lentivirus-mediated Genetic Manipulation and Visualization of Olfactory Sensory Neurons in vivo
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Cited by 7 •
2011
We present a lentiviral technique for genetic manipulation and visualization of single olfactory sensory neuron axon and its terminal arborization in vivo.
10:58
A Protocol for Lentiviral Transduction and Downstream Analysis of Intestinal Organoids
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Cited by 81 •
2015
In this video protocol we give a step by step explanation of lentiviral transduction in organoids of primary intestinal epithelium and of processing and downstream analysis of these cultures by quantitative RT-PCR, RNA-microarray and immunohistochemistry.
FAQs
After the vector binds receptors on the target cell, it enters the cell and releases its RNA cargo. Reverse transcription then converts that RNA into DNA, which can integrate into the host genome. This integrated DNA provides a basis for stable, long-term expression, making the approach useful when transient delivery would not provide a sufficient duration of gene activity.
The process supports long-term gene expression in cells regardless of whether they are actively dividing. This broad cellular range matters because biomedical studies and medical applications may involve different target-cell states. Researchers can therefore investigate gene function or pursue therapeutic strategies in systems where maintaining expression across a cell population, or within nondividing cells, is important.
Vector design and biosafety controls help manage risks associated with lentiviral use, including insertional and manufacturing risks. Because the delivered DNA can integrate into the host genome, design considerations are relevant to how genetic material is introduced and maintained. In research and medicine, these controls support more responsible development and evaluation of lentiviral applications.
Introducing genetic material into target cells allows investigators to examine how a gene affects cellular behavior or function. Stable expression is particularly useful when observations must extend beyond a short exposure period. This application makes lentiviral systems valuable for biomedical research that requires sustained gene activity while studying cellular mechanisms or evaluating the consequences of genetic changes.
The method can deliver genetic material to immune cells that are being engineered for therapeutic purposes. Sustained expression may help maintain the introduced genetic program during subsequent investigation or development. In medicine, this capability connects lentiviral delivery with engineered immune-cell therapies, while vector design and biosafety controls remain important parts of responsible application.
Lentiviral delivery provides a way to introduce genetic material into cells in the context of developing treatments for inherited or acquired diseases. Its capacity for long-term expression can support strategies that require a persistent genetic effect rather than temporary delivery. These applications remain linked to careful vector development and management of insertional and manufacturing risks.