Surface Glomeruli
Surface Glomeruli - Related Videos
03:35
Glomeruli Isolation: A Technique to Obtain Intact Glomeruli from Murine Kidney
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2023
This video describes the method to isolate intact glomeruli from mouse kidneys. The encapsulated and decapsulated glomeruli thus obtained can further be used for culturing parietal epithelial cell outgrowths from the murine kidney.
09:12
Isolation of Glomeruli and In Vivo Labeling of Glomerular Cell Surface Proteins
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Cited by 4 •
2019
Here we present a protocol for murine in vivo labeling of glomerular cell surface proteins with biotin. This protocol contains information on how to perfuse mouse kidneys, isolate glomeruli, and perform endogenous immunoprecipitation of the protein of interest.
12:19
Single-channel Analysis and Calcium Imaging in the Podocytes of the Freshly Isolated Glomeruli
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Cited by 25 •
2015
Changes in the intracellular calcium levels in the podocytes are one of the most important means to control the filtration function of glomeruli. Here we explain a high-throughput approach that allows detection of real-time calcium handling and single ion channels activity in the podocytes of the freshly isolated glomeruli.
10:14
An Efficient Sieving Method to Isolate Intact Glomeruli from Adult Rat Kidney
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Cited by 13 •
2018
The main focus of this protocol is to efficiently isolate viable primary glomeruli cultures with minimal contaminants for use in a variety of downstream applications. The isolated glomeruli retain structural relationships between component cell types and can be cultured ex vivo for a short time.
09:50
An Efficient and Fast Method for Labeling and Analyzing Mouse Glomeruli
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2024
This study presents an easy-to-use, complete, and simple set of methods to label and analyze glomeruli from CUBIC-cleared mouse kidneys. Data such as glomerulus number and volume can be obtained easily and reliably using fluorescein isothiocyanate (FITC)-Dextran, light sheet fluorescence microscopy (LSFM), or common confocal microscopy and software such as Imaris.
FAQs
Filtration depends on three coordinated layers: fenestrated capillary endothelium, the glomerular basement membrane, and podocyte slit diaphragms. Together, they permit water and small solutes to enter Bowman’s space while retaining cells and most proteins. Studying these components helps researchers distinguish normal filtration from changes associated with microvascular injury or proteinuric kidney disorders.
Blood pressure provides the driving force that moves water and small solutes across the glomerular filtration barrier. The resulting filtrate enters Bowman’s space, whereas cells and most proteins remain within the circulation. Measurements of this process can reveal how regional glomerular position relates to filtration and how injury may alter normal selectivity.
Their different cortical positions allow investigators to examine how nephron location influences renal structure and function. Comparing superficial and deeper, including juxtamedullary, glomeruli can identify regional differences in filtration or responses to injury. This comparison is useful because findings from one glomerular population may not represent the behavior of another.
The peripheral location of surface glomeruli can support direct imaging and physiological measurements. Investigators can examine accessible glomeruli while assessing filtration-related behavior or structural changes, then compare those observations with deeper glomerular populations. This approach connects visible microvascular features with functional measurements and helps evaluate regional patterns within the kidney.
They provide an accessible setting for examining glomerular capillaries and the filtration barrier during investigations of microvascular injury. Researchers can relate structural observations to changes in filtration, including altered movement of water or small solutes across the barrier. These measurements help characterize how injury affects glomerular function rather than assessing anatomy alone.
Proteinuric disorders can be investigated by examining how glomerular filtration normally retains most proteins and how that selectivity changes with disease. Surface glomeruli are useful because their position supports imaging and physiological assessment of this process. Comparing affected and deeper glomeruli may clarify regional contributions to abnormal protein passage and impaired renal function.