Glioblastoma Invasion
Glioblastoma Invasion - Related Videos
09:23
Coculture Assays to Study Macrophage and Microglia Stimulation of Glioblastoma Invasion
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Cited by 19 •
2016
Understanding the malignant behavior of cancer requires creating accurate models of how tumor cells interact with components of the tumor microenvironment, such as macrophages. Here we describe two methods to study glioblastoma cell interaction with tumor associated macrophages and microglia where the effect on glioblastoma invasion is assessed.
03:23
Matrix-Based Tumor Invasion Model Using Microglia Glioblastoma Co-Culture
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2025
In this video, glioblastoma and microglia cells are mixed with a polymer matrix solution and transferred to a porous chamber insert where a gel forms, embedding the cells. Microglia stimulate the migration of glioblastoma cells through the porous insert, mimicking tumor cell invasion into surrounding tissue, Cells are then fixed and prepared for further analysis.
08:35
A Human Glioblastoma Organotypic Slice Culture Model for Study of Tumor Cell Migration and Patient-specific Effects of Anti-Invasive Drugs
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Cited by 31 •
2017
Current ex vivo models of glioblastoma (GBM) are not optimized for physiologically relevant study of human tumor invasion. Here, we present a protocol for generation and maintenance of organotypic slice cultures from fresh human GBM tissue. A description of time-lapse microscopy and quantitative cell migration analysis techniques is provided.
02:29
Intravenous Administration of Therapeutic Nanoparticles in a Murine Glioblastoma Model
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2025
Source: Kim, B. D., et al. Nanoparticle Delivery of an Oligonucleotide Payload in a Glioblastoma Multiforme Animal Model. J. Vis. Exp. (2024)This video demonstrates the intravenous injection of therapeutic nanoparticles in a glioblastoma-bearing mouse. The method enables targeted delivery across the blood-brain barrier and supports non-invasive imaging for evaluating nanoparticle biodistribution and therapeutic efficacy in brain tumors.
02:36
Generating a Glioblastoma Rat Model
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2025
Source: Bolcaen, J., et. al., PET and MRI Guided Irradiation of a Glioblastoma Rat Model Using a Micro-irradiator. J. Vis. Exp. (2017)This video demonstrates the procedure for establishing a glioblastoma rat model by stereotactically injecting brain tumor-causing glioblastoma cells into the prefrontal cortex.
FAQs
A shift in cell adhesion helps malignant glial cells disengage from the primary tumor, while remodeling the actin cytoskeleton supports changes in cell shape and movement. These processes work together rather than independently: altered adhesion permits migration, and cytoskeletal restructuring helps cells navigate surrounding tissue. Their coordinated activity contributes to tumor spread beyond the main mass.
Tumor cells interact with the extracellular matrix, the structural material surrounding cells, as they move through brain tissue. Proteolytic enzymes can modify this surrounding environment, creating conditions that permit migration through tissue spaces. Studying both matrix interactions and enzyme activity helps researchers identify mechanisms that allow infiltrating cells to move beyond the visible tumor.
Signals from the brain microenvironment influence how tumor cells behave outside the primary mass. Their effects must be considered alongside adhesion changes, actin-cytoskeleton remodeling, extracellular-matrix interactions, and proteolytic activity. This broader view is important because invasion reflects communication between malignant cells and surrounding brain tissue, not solely properties intrinsic to the tumor cells.
Researchers examine invasion in patient-derived cultures, organoids, animal models, and live-cell imaging systems. Patient-derived cultures can preserve features from individual tumors, whereas organoids and animals provide more tissue-level context. Live-cell imaging adds direct observation of movement. Using these complementary systems helps connect cellular mechanisms with behavior in more complex environments.
Live-cell imaging allows researchers to observe tumor-cell movement as it occurs rather than relying only on an endpoint measurement. It can help relate changes in adhesion, cytoskeletal organization, matrix interaction, and responses to microenvironmental signals to actual migratory behavior. This information supports more precise analysis of how cells navigate surrounding tissue.
Because invasive cells can extend into tissue surrounding the primary mass, the visible tumor may not represent the full distribution of malignant cells. Research models that examine infiltration can improve understanding of this surrounding spread. That knowledge may inform surgical planning by emphasizing the challenge of removing the main mass while accounting for cells beyond its apparent boundaries.
Mechanistic studies identify pathways associated with adhesion, actin-cytoskeleton remodeling, extracellular-matrix interaction, proteolytic activity, and microenvironmental signaling. These pathways provide potential targets for approaches aimed at infiltrating cells as well as the main tumor. Such strategies are intended to address residual invasive cells, which are relevant to recurrence after treatment.