Mouse Brain Recording
Mouse Brain Recording - Related Videos
03:00
Recording of Local Field Potential from Both Hemispheres of the Mouse Brain
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2025
This video demonstrates in vivo recording of local field potential (LFP) from bilateral M2 areas of the mouse brain. An anesthetized mouse is secured and prepared for the procedure. To record LFPs, insert microelectrodes into the target coordinates of the bilateral M2 areas, which are responsible for complex motor movements.
05:36
Implantation of an Electrode Assembly in a Mouse Brain for Electrophysiological Recording
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2025
This video demonstrates a technique for implanting an electrode assembly into a mouse brain. The assembly contains bipolar electrodes to record activity from the hippocampus and a reference electrode to obtain baseline activity from the cerebellum. An anesthetized mouse is placed on a stereotaxic frame, and the electrode assembly is implanted in the target brain region. The animal is then allowed to recover before performing electrophysiological recording.
05:01
Injectable Mesh Electronics for Stable Single-Neuron Recordings in a Mouse Brain
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2025
The video demonstrates the injection of mesh electronics probes into a mouse brain for stable long-term recordings at a single-neuron level. An anesthetized mouse with an exposed skull is secured on a stereotaxic frame, and the probe's mesh device region is injected into the brain through drilled holes. The probe's input-output pads are connected to a circuit, and recordings are obtained using a data acquisition system.
02:14
Electrophysiological Recordings of Reprogrammed Neurons in a Mouse Brain Slice
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2025
This video demonstrates a protocol to evaluate the maturation and reprogramming of glial cells into neurons in a transfected mouse striatum slice. The experiment measures resting membrane potential and response to current injections using neuron-specific fluorescent markers and electrophysiological techniques. Voltage spikes during these measurements indicate successful neuronal maturation and reprogramming.
03:37
Recording Whole-Cell Currents with GABA Puffing in a Mouse Brain Slice
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2025
This video demonstrates the recording of whole-cell currents induced by gamma-aminobutyric acid, or GABA, in a mouse prefrontal cortical brain slice. Controlled delivery of GABA opens chloride channels in a postsynaptic neuron, generating inhibitory postsynaptic currents, or IPSCs. These IPSCs increase with higher GABA concentrations, reflecting GABA's inhibitory activity on synaptic transmission.
FAQs
Electrodes detect voltage changes produced by neuronal firing, whereas optical sensors track activity-linked calcium signals. These readouts provide complementary views of neural activity rather than identical measurements. Comparing them helps investigators select a signal appropriate to the question, whether they need evidence of firing-related electrical events or calcium dynamics associated with activity.
They can show how individual neurons and larger networks communicate across brain regions. This distinction matters because a single-cell measurement can identify activity patterns in particular neurons, while broader network recordings can indicate coordinated circuit behavior. In neuroscience, examining both scales helps connect local neuronal events with circuit-level processes supporting behavior, sensation, and cognition.
Behavioral measurements connect neural signals to what the mouse is doing or experiencing. This pairing allows researchers to ask whether activity changes accompany learning, memory, movement, or sensory processing instead of interpreting brain signals in isolation. The resulting relationship between circuit activity and behavior strengthens conclusions about how neural circuits support observable actions and cognitive functions.
Recordings can be used to examine how brain circuits change with experience or disease. Investigators may compare neural activity patterns in relation to these conditions and determine whether altered communication appears at the level of individual neurons or larger networks. This makes the approach useful for studying circuit mechanisms underlying neurological disorders, not merely cataloging activity.
An in vivo study combines neural measurement with observations of behavior. Electrodes may be used to detect voltage changes, or optical sensors may track calcium signals linked to activity, while behavioral measurements provide the experimental context. Together, these components produce a circuit-level view that relates recorded activity to sensation, cognition, movement, or learning and memory.
This approach is suited to questions about learning, memory, movement, and sensation, as well as the circuit mechanisms of neurological disorders. It can reveal whether relevant changes occur in individual neurons, across larger networks, or in communication between brain regions. Such findings help explain how activity patterns relate to behavior and disease-related circuit changes.
By exposing circuit mechanisms associated with behavior or neurological disorders, recordings provide evidence for evaluating potential therapeutic strategies. Researchers can use activity patterns and their relationship to behavior to judge whether a strategy is linked to changes in the relevant neural circuits. The method therefore supports mechanistic assessment, rather than relying only on behavioral outcomes.