Th Egfp Transgene
Th Egfp Transgene - Related Videos
02:16
Differentiating Non-Transgenic and Transgenic Human Pluripotent Stem Cells into Neural Progenitor Cells
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2025
This video demonstrates the generation of neural progenitor cells from non-transgenic and transgenic human pluripotent stem cells (hPSCs). hPSCs are differentiated into neural progenitor cells using a neuronal differentiation medium. While differentiation is induced in non-transgenic cells using small molecules, the transgenic hPSCs are differentiated by adding an antibiotic that induces the promoter for a neuronal differentiation-specific transcription factor.
10:15
Generation of Transgenic C. elegans by Biolistic Transformation
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Cited by 27 •
2010
Transgenic worms are commonly used in C. elegans research. Described is a simple, yet effective, protocol to introduce transgenes into worms using biolistic bombardment with DNA-coated gold particles. The effort involved and results of bombardment compare favorably with microinjection for the generation of transgenic animals.
08:19
Quantitative Comparison of cis-Regulatory Element (CRE) Activities in Transgenic Drosophila melanogaster
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Cited by 15 •
2011
Phenotypic variation for traits can result from mutations in cis-regulatory element (CRE) sequences that control gene expression patterns. Methods derived for use in Drosophila melanogaster can quantitatively compare the levels of spatial and temporal patterns of gene expression mediated by modified or naturally occurring CRE variants.
11:43
Easy Measurement of Diffusion Coefficients of EGFP-tagged Plasma Membrane Proteins Using k-Space Image Correlation Spectroscopy
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Cited by 37 •
2014
This paper provides a step by step guide to the fluctuation analysis technique k-Space Image Correlation Spectroscopy (kICS) for measuring diffusion coefficients of fluorescently labeled plasma membrane proteins in live mammalian cells.
10:03
Development of Leishmania Species Strains with Constitutive Expression of eGFP
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2023
Here, we describe the methodology used for generating L. panamensis and L. donovani strains expressing the gene for eGFP as a stable integrated transgene using the pLEXSY system. Transfected parasites were cloned by limiting dilution, and clones with the highest fluorescence intensity in both species were selected for further use in drug screening assays.
FAQs
The key control point is the regulatory sequence taken from the TH gene. When that regulatory program is active in a cell expressing tyrosine hydroxylase, it drives EGFP production in the same cellular context. Fluorescence therefore provides a genetically linked readout of TH-associated neuronal populations, allowing those cells to be distinguished from surrounding nervous-system tissue.
Fluorescent labeling highlights catecholaminergic populations associated with tyrosine hydroxylase, including dopaminergic and noradrenergic neurons. This distinction is useful because these populations participate in different nervous-system circuits and can be examined as genetically marked groups. The signal gives researchers a way to locate and study these neurons within nervous-system tissue rather than relying only on their surrounding anatomy.
EGFP fluorescence makes TH-expressing cells visually identifiable, linking a molecular characteristic to a specific cellular location. That linkage supports selective examination of the marked population during experiments on neuronal development, connectivity, and activity. It also helps researchers relate changes observed in particular neurons to broader alterations in catecholaminergic circuits.
Researchers can first identify fluorescent cells in nervous-system tissue and then focus measurements or manipulations on those marked neurons. The source material specifically supports targeted recording and cell isolation as uses of the construct. In practice, the fluorescent signal provides the selection criterion, helping experiments concentrate on TH-associated populations instead of nearby unmarked cells.
The labeled cells can be examined across several levels of neuroscience research. Their locations support visualization and circuit mapping, while their identities enable studies of connectivity and activity within catecholaminergic populations. Tracking these neurons during development can also reveal how relevant cell groups and circuits change over time.
The construct is particularly relevant when a study focuses on dopaminergic or noradrenergic neurons and the circuits they form. It can support investigations of neuronal development, connectivity, activity, and disease-related changes in those populations. By marking the relevant cells genetically, it helps connect cellular observations with changes in broader brain circuits.