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Journal / Neuroscience / SDS-PAGE/Immunoblot Detection of Aβ…
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Neuroscience

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JoVE Journal Neuroscience
SDS-PAGE/Immunoblot Detection of Aβ Multimers in Human Cortical Tissue Homogenates using Antigen-Epitope Retrieval
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SDS-PAGE/Immunoblot Detection of Aβ Multimers in Human Cortical Tissue Homogenates using Antigen-Epitope Retrieval

Article DOI: 10.3791/1916-v • 10:49 min • April 23rd, 2010
April 23rd, 2010 •
1, 2, 3, 1,4
1Yerkes National Primate Research Center, Emory University, 2Department of Neurology, Institute of Clinical Medicine, Tsukuba University, 3Department of Pathology, New York University School of Medicine, 4Department of Neurology, Emory University

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Summary
Automatic Translation

We describe a technique for the preparation of clarified human cortical homogenates, protein separation by SDS-PAGE, antigen retrieval and immunoblotting with an antibody to the Aβ peptide. Using this protocol, we consistently detect monomeric and multimeric Aβ in cortical tissue from humans with Alzheimer's pathology.

Tags

SDS-PAGE/Immunoblot Aβ Multimers Human Cortical Tissue Homogenates Antigen-epitope Retrieval Anomalous Folding Polymerization β-amyloid Peptide Neurodegenerative Cascade Alzheimer's Disease Pathogenesis Self-aggregation Amyloid Fibrils Cerebral Senile Plaques Low Molecular Weight Soluble Aβ Multimers Toxic Multimers Brain Tissue Proteopathies SDS-polyacrylamide Gel Electrophoresis Immunoblotting Aβ-specific Antibodies Highly Concentrated Cortical Homogenates Small Pore-size Nitrocellulose Membranes Clarified Human Cortical Homogenates
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