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Journal / Neuroscience / SDS-PAGE/Immunoblot检测人脑皮层组织中的Aβ多聚匀浆使用抗原表位检索…
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Neuroscience

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SDS-PAGE/Immunoblot检测人脑皮层组织中的Aβ多聚匀浆使用抗原表位检索
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SDS-PAGE/Immunoblot检测人脑皮层组织中的Aβ多聚匀浆使用抗原表位检索

Article DOI: 10.3791/1916-v • 10:49 min • April 23rd, 2010
April 23rd, 2010 •
1, 2, 3, 1,4
1Yerkes National Primate Research Center, Emory University, 2Department of Neurology, Institute of Clinical Medicine, Tsukuba University, 3Department of Pathology, New York University School of Medicine, 4Department of Neurology, Emory University

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Summary
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我们描述了一个准备的人脑皮层匀浆澄清，经SDS - PAGE分离蛋白，抗原修复，并与Aβ肽抗体免疫印迹技术。使用这个协议，我们一贯检测皮层组织与阿尔茨海默氏症的病理的人类单体和多聚体的Aβ。

Tags

朱庇特神经，第38期，β-淀粉样蛋白，低聚物，多聚体，免疫印迹，蛋白聚集，阿尔茨海默氏症，抗原修复
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