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Journal / Neuroscience / SDS-PAGE/Immunoblot Detecção de multímeros Aâ no…
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Neuroscience

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SDS-PAGE/Immunoblot Detecção de multímeros Aâ no tecido cortical Humanos homogeneizados utilizando antígeno Epitope-Retrieval

Article DOI: 10.3791/1916-v • 10:49 min • April 23rd, 2010
April 23rd, 2010 •
1, 2, 3, 1,4
1Yerkes National Primate Research Center, Emory University, 2Department of Neurology, Institute of Clinical Medicine, Tsukuba University, 3Department of Pathology, New York University School of Medicine, 4Department of Neurology, Emory University

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Nós descrevemos uma técnica para a preparação de esclarecer humanos homogeneizados cortical, separação de proteínas por SDS-PAGE, recuperação antigênica e immunoblotting com um anticorpo para o peptídeo Aâ. Usando este protocolo, que consistentemente detectar Aâ monomérica e multimérica no tecido cortical dos seres humanos com a patologia de Alzheimer.

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JOVE Neuroscience Edição 38 β-amilóide oligômeros multímeros Western blotting proteína de agregação a recuperação de Alzheimer antígeno,
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