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JoVE Journal Biology

Flow Cytometric Analysis of Mitophagy in Murine Pancreatic Î²-Cells Using the Genetically Encoded mt-Keima Reporter (Video) | JoVE

Purification of Native Complexes for Structural Study Using a Tandem Affinity Tag Method

Journal (Biology)

Purification of Native Complexes for Structural Study Using a Tandem Affinity Tag Method

Rapid and Efficient Generation of Neurons from Human Pluripotent Stem Cells in a Multititre Plate Format

Journal (Biology)

Rapid and Efficient Generation of Neurons from Human Pluripotent Stem Cells in a Multititre Plate Format

Flow Cytometric Analysis of Mitophagy in Murine Pancreatic Î²-Cells Using the Genetically Encoded mt-Keima Reporter (Video) | JoVE

Quantitative Analysis of Mitophagy in Murine Pancreatic β-Cells

Article DOI: 10.3791/200662-v • 01:45 min • September 15th, 2023

September 15th, 2023 •

Elena Levi-D’Ancona^1,2, Vaibhav Sidarala¹, Scott A. Soleimanpour^1,3,4

¹Department of Internal Medicine, Division of Metabolism, Endocrinology and Diabetes, University of Michigan, Ann Arbor, ²Graduate Program in Immunology, University of Michigan Medical School, ³Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, ⁴VA Ann Arbor Healthcare System

Tags

Mitophagy Flux Pancreatic Î²-cells Complementary Approaches Genetic Reporters Mt-Keima Mouse Model Quantifying Mitophagy Flow Cytometry PH-sensitive Dual-excitation Ratiometric Probe Acidic-to-neutral Mt-Keima Wavelength Emissions Genetic Mitophagy Reporters Complex Genetic Mouse Models Difficult-to-transfect Cells Primary Human Islets Dye-based Method MtPhagy Cell-permeable Dye Lysosomes During Mitophagy Fluozin-3-AM Zn2+ Indicator Tetramethylrhodamine Ethyl Ester (TMRE) Mitochondrial Membrane Potential

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