/

/

Quantitative Analysis of Mitophagy in Murine Pancreatic β-Cells

/

Flow Cytometric Analysis of Mitophagy in Murine Pancreatic β-Cells Using the Genetically Encoded mt-Keima Reporter

JoVE Journal
Biologie

Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.

JoVE Journal Biologie

Flow Cytometric Analysis of Mitophagy in Murine Pancreatic β-Cells Using the Genetically Encoded mt-Keima Reporter

Flow Cytometric Analysis of Mitophagy in Murine Pancreatic β-Cells Using the Genetically Encoded mt-Keima Reporter

Quantitative Analysis of Mitophagy in Murine Pancreatic β-Cells

DOI:

10.3791/200662-v

•

01:45 min

•

September 15, 2023

•

Elena Levi-D’Ancona², Vaibhav Sidarala, Scott A. Soleimanpour^3,4

¹Department of Internal Medicine, Division of Metabolism, Endocrinology and Diabetes,University of Michigan, Ann Arbor, ²Graduate Program in Immunology,University of Michigan Medical School, ³Department of Molecular and Integrative Physiology,University of Michigan, Ann Arbor, ⁴VA Ann Arbor Healthcare System

Tags

Flow Cytometry Mitophagy Pancreatic Beta Cells Mt-Keima Reporter FluoZin-3-AM DAPI Single Cell Analysis Gating Strategy Valinomycin Mitophagy Flux

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

Author Spotlight: Assessment of Mitophagy Flux in Pancreatic β-Cells Using Effective and Robust Complementary Approaches

Single-Cell Dissociation of Murine Islets for Flow Cytometric Analysis of β-Cell Mitophagy

Flow Cytometric Analysis of Mitophagy in Murine Pancreatic β-Cells Using MtPhagy Dye

Flow Cytometric Analysis of Mitophagy in Murine Pancreatic β-Cells Using the Genetically Encoded mt-Keima Reporter

Read Article