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Journal / Immunology and Infection / Icke-kirurgisk intratrakeal instillation av möss…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Icke-kirurgisk intratrakeal instillation av möss med analys av lungor och Lungsjukas Tömning lymfkörtlar med flödescytometri
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Icke-kirurgisk intratrakeal instillation av möss med analys av lungor och Lungsjukas Tömning lymfkörtlar med flödescytometri

Article DOI: 10.3791/2702-v • 08:02 min • May 2nd, 2011
May 2nd, 2011 •
1, 2, 1, 3, 1,2,4, 1,4
1Department of Immunology, University of Colorado School of Medicine, 2Division of Cell Biology, Department of Pediatrics, National Jewish Health, 3Department of Microbiology, Immunology, and Pathology, Colorado State University, 4Department of Immunology, National Jewish Health

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Vi illustrerar icke-kirurgisk leverans av provmaterial i lungorna på sövda möss via luftstrupen. Denna metod medger lung exponering mot bakteriella och virala patogener, cytokiner, antikroppar, pärlor, kemikalier eller färgämnen. Vi ytterligare beskriva skörd och förädling av lungorna och lungor dränerande lymfkörtlar (LDLNs) för flödescytometri.

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Immunologi 51 intratrakeal mus lungor lungor dränerande lymfkörtlar flödescytometri
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