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Journal / Bioengineering / 使用电化学传感器的细菌检测和鉴定
JoVE Journal
Bioengineering

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使用电化学传感器的细菌检测和鉴定
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使用电化学传感器的细菌检测和鉴定

Article DOI: 10.3791/4282-v • 09:30 min • April 23rd, 2013
April 23rd, 2013 •
1,2, 3, 2, 2,4,5
1Research Service, Veterans Affairs Greater Los Angeles Healthcare System, 2Department of Urology, The David Geffen School of Medicine, University of California, Los Angeles, 3GeneFluidics, 4Division of Infectious Diseases, Veterans Affairs Greater Los Angeles Healthcare System, 5Department of Microbiology, Immunology & Molecular Genetics, University of California, Los Angeles

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我们描述了一种电化学传感器的测定方法，细菌的快速检测和识别。的测定法涉及官能化的传感器阵列与DNA的核糖体RNA（rRNA基因）的种属特异性序列的寡核苷酸捕捉探针。目标rRNA基因的捕获探针和辣根过氧化物酶相连的DNA寡核苷酸检测探针的夹心杂交产生可测量的安培电流。

Tags

生物工程，第74期，微生物学，遗传学，分子生物学，细胞生物学，生物化学，生物医学工程，医学，免疫学，细菌，电化学传感器，核糖体RNA，rRNA基因，16S RNA，DNA，探针，检测
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