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Journal / Bioengineering / 成比例的生物传感器测量活酵母细胞中的线粒体氧化还原状态和ATP…
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Bioengineering

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成比例的生物传感器测量活酵母细胞中的线粒体氧化还原状态和ATP

Article DOI: 10.3791/50633-v • 12:22 min • July 22nd, 2013
July 22nd, 2013 •
*1, *1, 2, 2, 1
1Department of Pathology and Cell Biology, Columbia University, 2Herbert Irving Comprehensive Cancer Center, Columbia University
* These authors contributed equally

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Summary
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我们描述了使用两个比例，基因编码生物传感器的，这是基于GFP，监测线粒体的氧化还原状态和细胞内ATP水平分辨率在活酵母细胞。

Tags

生物工程，77期，微生物学，细胞生物学，分子生物学，生物化学，生命科学，roGFP，氧化还原敏感的绿色荧光蛋白，GO-ATEAM，ATP，ROS，线粒体，生物传感器，GFP，ImageJ的，显微镜，激光共聚焦显微镜，荧光共振能量转移（FRET），细胞，成像
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