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Journal / Bioengineering / Biosensori MIL Tale misura mitocondriale stato…
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Biosensori MIL Tale misura mitocondriale stato redox e ATP in cellule viventi di lievito
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Biosensori MIL Tale misura mitocondriale stato redox e ATP in cellule viventi di lievito

Article DOI: 10.3791/50633-v • 12:22 min • July 22nd, 2013
July 22nd, 2013 •
*1, *1, 2, 2, 1
1Department of Pathology and Cell Biology, Columbia University, 2Herbert Irving Comprehensive Cancer Center, Columbia University
* These authors contributed equally

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Descriviamo l'uso di due raziometrici, biosensori geneticamente codificati, che si basano sulla GFP, per monitorare lo stato redox mitocondriale e livelli di ATP a risoluzione subcellulare in vita cellule di lievito.

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Bioingegneria Numero 77 Microbiologia Biologia Cellulare Biologia Molecolare Biochimica scienze della vita roGFP proteina fluorescente verde redox-sensibili GO-Ateam ATP FRET ROS i mitocondri biosensori GFP ImageJ microscopia microscopia confocale cella imaging
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