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Journal / Bioengineering / En forbedret fremgangsmåte for fremstillingen av…
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Bioengineering

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JoVE Journal Bioengineering
En forbedret fremgangsmåte for fremstillingen av type I kollagen fra hud
Evaluating Primary Blast Effects In Vitro
Journal (Bioengineering)
Evaluating Primary Blast Effects In Vitro
Quantifying the Mechanical Properties of the Endothelial Glycocalyx with Atomic Force Microscopy
Journal (Bioengineering)
Quantifying the Mechanical Properties of the Endothelial Glycocalyx with Atomic Force Microscopy
 
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En forbedret fremgangsmåte for fremstillingen av type I kollagen fra hud

Article DOI: 10.3791/51011-v • 05:17 min • January 21st, 2014
January 21st, 2014 •
1,2, 1, 1,2
1Department of Anesthesiology, Perioperative and Pain Medicine, Boston Children's Hospital, 2Department of Anesthesia, Harvard Medical School

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Tradisjonelle prosedyrer for isolering av løselig type 1 kollagen (kol1) krever omtrent 10 dager fra start til slutt på grunn av lange buffer inkubasjoner og arbeidskrevende resuspensions av fibriller. Her beskriver vi et middel for å rense kol1 fra små dermal biopsier på mindre enn tre timer.

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Bioteknologi type 1 kollagen ekstracellulære matrise tissue engineering scaffoldprotein dermis corium
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