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Journal / Biology / Protein-protein interaktioner visualiseret ved…
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Protein-protein interaktioner visualiseret ved bimolekylær Fluorescens komplementering i tobaksprotoplaster og blade

Article DOI: 10.3791/51327-v • 11:10 min • March 9th, 2014
March 9th, 2014 •
1, 1
1Department Biologie I, Botanik, Ludwig-Maximilians-Universität, München

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Dannelse af protein-komplekser in vivo kan visualiseres ved bimolekylær fluorescens komplementering. Interaktionspartnere fusioneres til komplementære dele af fluorescerende tags og udtrykt transient i tobaksblade, hvilket resulterer i et rekonstitueret fluorescenssignal ved tæt nærhed af de to proteiner.

Tags

Plantebiologi Tetratricopeptide gentage domæne chaperone kloroplaster endoplasmatiske reticulum HSP90 Toc kompleks Sec translocon BiFC
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