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In tempo reale Imaging del singolo Engineered RNA trascritti in cellule viventi Utilizzando Ratiometric bimolecolari Beacons

Article DOI: 10.3791/51544-v • 12:20 min • August 6th, 2014
August 6th, 2014 •
*1, *1, 2, 2, 1
1Department of Bioengineering, University of Pennsylvania, 2Integrated DNA Technologies, Inc.
* These authors contributed equally

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Ratiometric beacon bimolecolari (RBMBs) possono essere utilizzati per immagini singole trascritti di RNA ingegnerizzati in cellule viventi. Qui, descriviamo la preparazione e purificazione di RBMBs, consegna delle RBMBs in cellule di microporation e l'imaging fluorescente di trascritti di RNA singoli in tempo reale.

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Genetica RNA immagini singola molecola fluorescenza cellula vivente
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