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Realtid avbildning av Single Engineered RNA transkript hos levande celler Använda Propportionell bimolekylär Beacons

Article DOI: 10.3791/51544-v • 12:20 min • August 6th, 2014
August 6th, 2014 •
*1, *1, 2, 2, 1
1Department of Bioengineering, University of Pennsylvania, 2Integrated DNA Technologies, Inc.
* These authors contributed equally

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Ratiometrisk bimolekylära fyrar (RBMBs) kan användas för att avbilda enskilda manipulerade RNA-transkript i levande celler. Här beskriver vi framställning och rening av RBMBs, leverans av RBMBs in i celler genom mikroporation och fluorescerande avbildning av enstaka RNA-transkript i realtid.

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Genetik RNA imaging enda molekyl fluorescens levande cell
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