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Immunology and Infection

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Anvendelse fluorescensresonansenergioverførsel (FRET) til at undersøge Effector translokationseffektivitet af
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Anvendelse fluorescensresonansenergioverførsel (FRET) til at undersøge Effector translokationseffektivitet af

Article DOI: 10.3791/54210-v • 10:29 min • July 6th, 2016
July 6th, 2016 •
1, 1, 1
1Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne

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Undersøge samspillet mellem bakterielle patogener og deres værter er et vigtigt område for biologisk forskning. Her beskriver vi de nødvendige teknikker til at måle effektor translokation af Coxiella burnetii under siRNA gendæmpning hjælp BlaM substrat.

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Infektion Host-patogen interaktioner bakterielle sekretion systemer β-lactamase translokation assay BlaM substrat fluorescens mikrobiologi intracellulære bakterielle patogener, Gene silencing siRNA molekylærbiologi
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