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JoVE Journal Cancer Research

에 약물 저항 관련 소설 스플 라이스 변종을 감지하는 RNA 시퀀싱을 사용하여

Monitoring Breast Cancer Growth and Metastatic Colony Formation in Mice using Bioluminescence

Journal (Cancer Research)

Monitoring Breast Cancer Growth and Metastatic Colony Formation in Mice using Bioluminescence

Studying the Effects of Tumor-Secreted Paracrine Ligands on Macrophage Activation using Co-Culture with Permeable Membrane Supports

Journal (Cancer Research)

Studying the Effects of Tumor-Secreted Paracrine Ligands on Macrophage Activation using Co-Culture with Permeable Membrane Supports

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에 약물 저항 관련 소설 스플 라이스 변종을 감지하는 RNA 시퀀싱을 사용하여

Article DOI: 10.3791/54714-v • 09:58 min • December 9th, 2016

December 9th, 2016 •

Rocco Sciarrillo^1,2,3, Anna Wojtuszkiewicz¹, Irsan E. Kooi⁴, Valentina E. Gómez³, Ugo Boggi⁵, Gerrit Jansen⁶, Gert-Jan Kaspers^1,7, Jacqueline Cloos*¹, Elisa Giovannetti*^3,8,9

¹Department of Pediatric Oncology/Hematology, VU University Medical Center, ²Department of Hematology, VU University Medical Center, ³Department of Medical Oncology, VU University Medical Center, ⁴Department of Clinical Genetics, VU University Medical Center, ⁵Division of General and Transplant Surgery, Azienda Ospedaliera Universitaria Pisana, Universita’ di Pisa, ⁶Amsterdam Immunology and Rheumatology Center, VU University Medical Center, ⁷Princess Máxima Center for Pediatric Oncology, ⁸Cancer Pharmacology Lab, AIRC Start-Up Unit, University of Pisa, ⁹Institute of Nanoscience and Nanotechnology, CNR-Nano

* These authors contributed equally

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여기에서 우리는 고형 종양 및 혈액 악성 종양에서 약물 내성에 비정상적인 스 플라이 싱의 영향을 조사하기위한 프로토콜을 설명합니다. 이 목표를 위해, 우리는 RNA-서열을 통해 부모와 체외에서 저항 모델의 transcriptomic 프로필을 분석 후보 유전자를 확인하기 위해 QRT-PCR 기반의 방법을 설립했다.

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암 연구 문제 (118) 화학 요법 저항 세포 독성 대체 접합 RNA-SEQ transcriptomics

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