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Bioengineering

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Um sistema integrado para acionar remotamente a transdução do sinal intracelular por Photoactivation Upconversion Nanoparticle-mediada da quinase

Article DOI: 10.3791/55769-v • 11:20 min • August 30th, 2017
August 30th, 2017 •
1,2, 1, 1,2, 3, 1,2, 1
1Institute of Chemistry, Academia Sinica, 2Department of Chemistry, National Taiwan University, 3Department of Materials and Mineral Resources Engineering, National Taipei University of Technology

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Neste protocolo, enjaulado proteína quinase A (PKA), um bioeffector de transdução de sinal de celular, foi imobilizada em uma superfície de nanopartículas, injetada no citosol e ativada pelo upconverted UV luz de irradiação de infravermelho próximo (NIR), induzindo desintegração de fibra de stress a jusante no citosol.

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Bioengenharia edição 126 enzima Caged resposta celular microinjeção fotólise de proteína da upconversion nanopartículas photoactivation
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