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Bioengineering

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Isolering av primära murin retinala Ganglion-celler (RGC) genom flödescytometri

Article DOI: 10.3791/55785-v • 11:01 min • July 5th, 2017
July 5th, 2017 •
1, 1, 1, 1, 1, 2, 1,3,4, 1,2
1Department of Ophthalmology, Hamilton Eye Institute, University of Tennessee Health Science Center, 2Department of Microbiology, Immunology and Biochemistry, University of Tennessee Health Science Center, 3Department of Anatomy and Neurobiology, University of Tennessee Health Science Center, 4Department of Pharmaceutical Sciences, University of Tennessee Health Science Center

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Miljoner människor lider av retinal degenerativa sjukdomar som leder till irreversibel blindhet. Ett vanligt element i många av dessa sjukdomar är förlusten av retinala ganglionceller (RGC). Detta detaljerade protokoll beskriver isoleringen av primära murin RGC genom positivt och negativt urval med flödescytometri.

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Bioengineering retinal ganglion celler RGC flödescytometri neurodegeneration glaukom åldrande
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