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Genetics

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JoVE Journal Genetics
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Genomgående kartläggning av protein-DNA-interaktioner med ChEC-seq in

Article DOI: 10.3791/55836-v • 10:43 min • June 3rd, 2017
June 3rd, 2017 •
1, 2
1Basic Sciences Division, Fred Hutchinson Cancer Research Center, 2Department of Biology, Indiana University

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Vi beskriver kromatin endogen klyvning i kombination med hög genomströmnings-sekvensering (ChEC-seq), en kromatinimmunpresipitations (ChIP) -orthogonal metod för kartläggning av proteinbindningsställen genomgående med mikrokocknukleas (MNase) fusionsproteiner.

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Genetics ChEC-seq ChIP-seq transkription genomomfattande Mediator,
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