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Journal / Developmental Biology / En RANKL-baserte Osteoclast kultur analysen av…
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Developmental Biology

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JoVE Journal Developmental Biology
En RANKL-baserte Osteoclast kultur analysen av musen benmarg å undersøke rollen som mTORC1 i Osteoclast-formasjonen
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En RANKL-baserte Osteoclast kultur analysen av musen benmarg å undersøke rollen som mTORC1 i Osteoclast-formasjonen

Article DOI: 10.3791/56468-v • 09:37 min • March 15th, 2018
March 15th, 2018 •
*1, *2, *1, 3, 2, 1, 2, 1
1Department of Pediatric Dentistry, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, National Clinical Research Center of Stomatology, 2State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, University of Chinese Academy of Sciences, 3Department of Oral and Maxillofacial-Head and Neck Oncology, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine
* These authors contributed equally

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Dette manuskriptet beskriver en protokoll for å isolere og kultur osteoklast i vitro fra musen benmargen, og å studere rollen av pattedyr/mekanistisk målet på rapamycin komplekse 1 i osteoclast-formasjonen.

Tags

Utviklingspsykologi biologi problemet 133 Osteoclast mTORC1 Raptor bein remolding cellebiologi RANKL M-CSF
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