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Journal / Developmental Biology / Encellede kvantificering af Protein nedbrydning…
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Developmental Biology
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Encellede kvantificering af Protein nedbrydning satser for Time-Lapse Fluorescens mikroskopi i vedhængende cellekultur

Article DOI: 10.3791/56604-v • 12:07 min • February 4th, 2018
February 4th, 2018 •
1, 1
1UPSUTER, Institute of Bioengineering (IBI), School of Life Sciences, École Polytechnique Fédérale de Lausanne (EPFL)

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Denne protokol beskriver en metode til at bestemme protein halveringstider i enkelt levende vedhængende celler, ved hjælp af pulse mærkning og fluorescens time-lapse billeddannelse af SNAP-tag fusion proteiner.

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Udviklingsmæssige biologi sag 132 Protein nedbrydning SNAP-tag enkelt celler vedhængende cellekultur mus embryonale stamceller Fluorescens mikroskopi live celle imaging puls mærkning fluorescerende farvestof
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