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Journal / Developmental Biology / Cella singola quantificazione dei tassi di…
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Developmental Biology
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Cella singola quantificazione dei tassi di degradazione delle proteine mediante microscopia a fluorescenza time-lapse in coltura delle cellule aderenti

Article DOI: 10.3791/56604-v • 12:07 min • February 4th, 2018
February 4th, 2018 •
1, 1
1UPSUTER, Institute of Bioengineering (IBI), School of Life Sciences, École Polytechnique Fédérale de Lausanne (EPFL)

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Questo protocollo descrive un metodo per determinare la proteina emivite in singole cellule aderenti, utilizzando impulsi etichettatura e time-lapse imaging di fluorescenza di proteine di fusione SNAP-tag.

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Biologia dello sviluppo problema 132 degradazione proteica SNAP-tag singole cellule cellule staminali embrionali di topo coltura delle cellule aderenti microscopia a fluorescenza cella immagini dal vivo etichettatura tintura fluorescente di impulso
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