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En kinetisk fluorescens-baserede Ca2 + mobilisering Assay at identificere G Protein-koblet Receptor agonister og antagonister allosteriske modulatorer

Article DOI: 10.3791/56780-v • 07:41 min • February 20th, 2018
February 20th, 2018 •
1, 1, 1, 1, 1
1Laboratory of Virology and Chemotherapy, Department of Microbiology and Immunology, Rega Institute for Medical Research, KU Leuven

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Den beskrevne cellulære assay er designet til identifikation af CXC chemokine receptor 4 (CXCR4)-interagerende agenter, der hæmmer eller stimulere enten konkurrencedygtige eller allosterically, den intracellulære Ca2 + release initieret af CXCR4 aktivering.

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Medicin sag 132 G protein-koblet receptor chemokine CXC chemokine receptor 4 fluorescens celle-baserede assay Ca2 + mobilisering agonist antagonist drug discovery allosteriske modulator små molekyler
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