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Journal / Immunology and Infection / Mus - og menneske-afledte primære gastrisk…
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Immunology and Infection

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Mus - og menneske-afledte primære gastrisk epitelial éncellelag kultur for studiet af regenerering
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Mus - og menneske-afledte primære gastrisk epitelial éncellelag kultur for studiet af regenerering

Article DOI: 10.3791/57435-v • 11:48 min • May 7th, 2018
May 7th, 2018 •
1, 2, 1, 3, 1
1Department of Pharmacology and Systems Physiology, University of Cincinnati, 2Department of Cell & Developmental Biology, University of Michigan, 3Department of Molecular Genetics, Biochemistry & Microbiology, University of Cincinnati

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Her beskriver vi en protokol til oprettelse og dyrkning menneskelige - og mus-afledte 3-dimensionelle (3D) gastrisk organoids, og metoden for overførslen af 3D organoids til en 2-dimensional éncellelag. Brug af gastrisk epitelial éncellelag som en roman scratch-sår assay for regenerering undersøgelser er også beskrevet.

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Immunologi og infektion spørgsmålet 135 scratch-sår assay gastrisk organoids stamceller reparation regenerering epithelial éncellelag
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