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Journal / Biology / Split grønne fluorescerende Protein System å…
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Split grønne fluorescerende Protein System å visualisere effektor levert fra bakterier under infeksjon

Article DOI: 10.3791/57719-v • 07:25 min • May 24th, 2018
May 24th, 2018 •
1, 1, 2, 1, 1
1Department of Plant Science, Plant Genomics and Breeding Institute, College of Agriculture and Life Science, Seoul National University, 2Department of Bioindustry and Bioresource Engineering, Sejong University

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Fluorescerende protein-baserte tilnærminger til overvåke effektor utskilles av bakterier i vertsceller er utfordrende. Dette skyldes inkompatibilitet mellom fluorescerende proteiner og type-III sekresjon systemet. Her, brukes en optimalisert delt superfolder GFP system for visualisering av effektor utskilles av bakterier i verten anlegget celle.

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Biologi problemet 135 Type III sekresjon system effektor dynamikk Pseudomonas superfolder grønne fluorescerende protein system subcellular lokalisering
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