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Journal / Biology / 秀丽线虫绿色荧光蛋白标记和 4…
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JoVE Journal Biology
秀丽线虫绿色荧光蛋白标记和 4 '、6-Diamidino-2-苯基吲哚染色检测蛋白质亚细胞定位
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秀丽线虫绿色荧光蛋白标记和 4 '、6-Diamidino-2-苯基吲哚染色检测蛋白质亚细胞定位

Article DOI: 10.3791/57914-v • 09:36 min • July 30th, 2018
July 30th, 2018 •
1, 1, 1
1Department of Science, Borough of Manhattan Community College/CUNY

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该协议演示了如何在热应激下跟踪蛋白质核易位, 方法是使用绿色荧光蛋白 (GFP) 融合蛋白作为标记, 4 ', 6-diamidino-2-苯基吲哚 (DAPI) 染色。DAPI 染色协议快速, 保留了 GFP 和蛋白质亚细胞定位信号。

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生物学 问题 137 热应激 亚细胞定位变化 核定位 绿色荧光蛋白 DAPI 染色 乙醇固定 丙酮固定
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