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Investigating Mammalian Axon Regeneration
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Investigating Mammalian Axon Regeneration: In Vivo Electroporation of Adult Mouse Dorsal Root Ganglion

Article DOI: 10.3791/58171-v • 06:17 min • September 1st, 2018
September 1st, 2018 •
1, 1, 1,2
1Department of Orthopaedic Surgery, Johns Hopkins University School of Medicine, 2The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine

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Summary
Automatic Translation

Electroporation is an effective approach to deliver genes of interests into cells. By applying this approach in vivo on the neurons of adult mouse dorsal root ganglion (DRG), we describe a model to study axon regeneration in vivo.

Tags

In-vivo Electroporation Adult Mouse Dorsal Root Ganglion Gene Transfection Gene Expression Manipulation Exo-regeneration Iliac Crest Marking Incision Muscle Detachment Fascia Joint Exposure Neural Arch Removal DRG Injection DNA Plasmids RNA Oligos Micro Injection Dispense System Electroporation
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