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Journal / Immunology and Infection / Forskelsbehandling af syv immun celle Subsets ved …
JoVE Journal
Immunology and Infection

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Forskelsbehandling af syv immun celle Subsets ved to-fluorokrom Flow flowcytometri

Article DOI: 10.3791/58955-v • 10:58 min • March 5th, 2019
March 5th, 2019 •
1, 2
1Oncology Research, Medimmune, LLC, 2Division of Rheumatology, Johns Hopkins University School of Medicine

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Her præsenterer vi et flow cytometric protokol for at identificere CD4+ og CD8+ T celler, γδ T celler, B-celler, NK-celler og monocytter i humant perifert blod ved hjælp af kun to fluorokromer i stedet for syv. Med denne tilgang, kan fem ekstra markører registreres på de fleste flow cytometers.

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Immunologi og infektion sag 145 flowcytometri immunologi Immunofænotypning humant perifert blod fluorokrom patient multiparametric analyse.
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