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Journal / Immunology and Infection / Discriminação de sete subconjuntos de células…
JoVE Journal
Immunology and Infection

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Discriminação de sete subconjuntos de células imunes por dois-fluorocromo citometria de fluxo

Article DOI: 10.3791/58955-v • 10:58 min • March 5th, 2019
March 5th, 2019 •
1, 2
1Oncology Research, Medimmune, LLC, 2Division of Rheumatology, Johns Hopkins University School of Medicine

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Aqui, apresentamos um protocolo de fluxo cytometric para identificar CD4+ e CD8+ T células, as células γδ T, células B, células NK e monócitos no sangue periférico humano usando apenas dois fluorochromes em vez de sete. Com esta abordagem, cinco marcadores adicionais podem ser gravadas na maioria dos citômetros.

Tags

Imunologia e infecção edição 145 citometria de fluxo imunologia immunophenotyping sangue periférico humano fluorocromo paciente Multiparamétrico de análise.
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