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JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Discriminazione dei sette sottoinsiemi delle cellule immuni di due-fluorocromo flusso Cytometry
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Non-Invasive Model of Neuropathogenic Escherichia coli Infection in the Neonatal Rat
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Discriminazione dei sette sottoinsiemi delle cellule immuni di due-fluorocromo flusso Cytometry

Article DOI: 10.3791/58955-v • 10:58 min • March 5th, 2019
March 5th, 2019 •
1, 2
1Oncology Research, Medimmune, LLC, 2Division of Rheumatology, Johns Hopkins University School of Medicine

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Qui, presentiamo un protocollo di cytometric di flusso per identificare CD4+ e CD8+ T cellule, γδ T, cellule B, cellule NK e monociti nel sangue periferico umano utilizzando solo due fluorocromi anziché sette. Con questo approccio, cinque ulteriori marcatori possono essere registrati sulla maggior parte dei citometri a flusso.

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Immunologia e infezione problema 145 citometria a flusso immunologia immunofenotipizzazione sangue periferico umano fluorocromo analisi multiparametrica paziente.
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