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Immunology and Infection

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Co-staining Blood Vessels and Nerve Fibers in Adipose Tissue

Article DOI: 10.3791/59266-v • 12:05 min • February 13th, 2019
February 13th, 2019 •
1, 2, 1, 1
1Center for Metabolic and Degenerative Diseases, the Brown Foundation of Institute of Molecular Medicine, University of Texas Health Science Center at Houston, 2Microscopy Core, the Brown Foundation of Institute of Molecular Medicine, University of Texas Health Science Center at Houston

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Summary
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New blood vessel formation and sympathetic innervation play pivotal roles in adipose tissue remodeling. However, there remain technical issues in visualizing and quantitatively measuring adipose tissue. Here we present a protocol to successfully label and quantitatively compare the densities of blood vessels and nerve fibers in different adipose tissues.

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Co-staining Blood Vessels Nerve Fibers Adipose Tissue Angiogenesis Sympathetic Innovation Immunofluorescence Method Confocal Microscopy High Resolution Images Blood Vessel Networks Characteristics Analysis Anesthesia Perfusion C57 Black-6J Male Mice White Adipose Tissue Brown Adipose Tissue Tissue Embedding Cassettes Fixation Solution Petri Dish Wash PBS Buffer Permeabilization
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