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Developmental Biology

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JoVE Journal Developmental Biology

臍帯血由来から 3 D 肌 Organoid の生成誘導多能性幹細胞

Efficient Generation of hiPSC Neural Lineage Specific Knockin Reporters Using the CRISPR/Cas9 and Cas9 Double Nickase System

Journal (Developmental Biology)

Efficient Generation of hiPSC Neural Lineage Specific Knockin Reporters Using the CRISPR/Cas9 and Cas9 Double Nickase System

Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation

Journal (Developmental Biology)

Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation

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臍帯血由来から 3 D 肌 Organoid の生成誘導多能性幹細胞

Article DOI: 10.3791/59297-v • 09:54 min • April 18th, 2019

April 18th, 2019 •

Yena Kim^1,2, Ji Hyeon Ju^1,2,3

¹CiSTEM Laboratory, Catholic Induced Pluripotent Stem Cell (iPSC) Research Center, College of Medicine, The Catholic University of Korea, ²Department of Biomedicine & Health Science, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, ³Division of Rheumatology, Department of Internal Medicine, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea

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これらのケラチノサイトと線維芽細胞を用いた誘導多能性幹細胞由来ケラチノサイトと線維芽細胞を区別し、3 D 肌 organoid を生成する方法を示すプロトコルを提案します。このプロトコルには、ヒト化マウスモデルを生成する追加の手順が含まれています。ここで紹介しているテクニックは、皮膚の研究が改善されます。

Tags

発生生物学、問題 146、誘導多能性幹細胞、臍帯血単核細胞、線維芽細胞、ケラチノサイト、3 D 皮膚における、皮膚移植、ヒト化マウスモデル

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