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Journal / Cancer Research / Flow Cytometrisk analyse af mitokondrie reaktive…
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JoVE Journal Cancer Research
Flow Cytometrisk analyse af mitokondrie reaktive oxygen arter i murine hæmatopoietiske stamceller og Progenitorcellerne og MLL-AF9 drevet leukæmi
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Flow Cytometrisk analyse af mitokondrie reaktive oxygen arter i murine hæmatopoietiske stamceller og Progenitorcellerne og MLL-AF9 drevet leukæmi

Article DOI: 10.3791/59593-v • 09:44 min • September 5th, 2019
September 5th, 2019 •
1, 1
1Blood Cell Development and Function Program, Fox Chase Cancer Center

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Vi beskriver en metode til brug af multiparametrflow cytometri til at detektere mitokondrie reaktive oxygenarter (ROS) i murine sund hæmatopoietisk stamcelle-og progenitorceller (Hspc'er) og leukæmiceller fra en musemodel af akut myeloid leukæmi (AML) drevet af MLL-AF9.

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Kræftforskning AML HSCs ROS super oxider mitochondrier flow flowcytometer
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