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Journal / Genetics / 通过旗-生物素串联纯化通过交叉链接和免疫沉淀对蛋白质-RNA相互作用进行转录组-全过程分析…
JoVE Journal
Genetics

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JoVE Journal Genetics
通过旗-生物素串联纯化通过交叉链接和免疫沉淀对蛋白质-RNA相互作用进行转录组-全过程分析
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通过旗-生物素串联纯化通过交叉链接和免疫沉淀对蛋白质-RNA相互作用进行转录组-全过程分析

Article DOI: 10.3791/60730-v • 08:46 min • May 18th, 2020
May 18th, 2020 •
1,2, 1, 1
1Tsinghua-Peking Center for Life Sciences, School of Medicine and School of Life Sciences, Tsinghua University, 2Department of Molecular Biology, University of Texas Southwestern Medical Center

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在这里，我们提出了一个改进的 CLIP-seq 协议， 称为 FbioCLIP-seq 与 FLAG 生物素串联纯化，以确定哺乳动物细胞中RNA结合蛋白（RBPs）的RNA靶点。

Tags

遗传学， 问题 159， FbioCLIP-seq， RNA 生物学， RNA 结合蛋白， 蛋白质 - RNA 相互作用， WDR43， LIN28
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