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Journal / Genetics / Transcriptome-Wide Profilering van Protein-RNA…
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Genetics

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JoVE Journal Genetics
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Transcriptome-Wide Profilering van Protein-RNA Interacties door Cross-Linking en Immunoprecipitatie Gemedieerd door FLAG-Biotine Tandem Purification

Article DOI: 10.3791/60730-v • 08:46 min • May 18th, 2020
May 18th, 2020 •
1,2, 1, 1
1Tsinghua-Peking Center for Life Sciences, School of Medicine and School of Life Sciences, Tsinghua University, 2Department of Molecular Biology, University of Texas Southwestern Medical Center

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Hier presenteren we een gewijzigd CLIP-seq protocol genaamd FbioCLIP-seq met FLAG-biotine tandem zuivering om de RNA doelen van RNA-bindende eiwitten (RBPs) in zoogdiercellen te bepalen.

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Genetica FbioCLIP-seq RNA biologie RNA-bindend eiwit eiwit-RNA interactie WDR43 LIN28
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