Conjugative Mating Assays for Sequence-specific Analysis of Transfer Proteins Involved in Bacterial Conjugation

Fettah Erdogan; Cristina Lento; Ayat Yaseen; Roksana Nowroozi-Dayeni; Sasha Kheyson; Gerald F. Audette

doi:10.3791/54854

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Konjugative Mating Assays für sequenzspezifische Analyse der Übertragung beteiligten Proteine in bakteriellen Konjugation

Conjugative Mating Assays for Sequence-specific Analysis of Transfer Proteins Involved in Bacterial Conjugation

JoVE Journal
Genetik

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JoVE Journal Genetik

Conjugative Mating Assays for Sequence-specific Analysis of Transfer Proteins Involved in Bacterial Conjugation

Konjugative Mating Assays für sequenzspezifische Analyse der Übertragung beteiligten Proteine in bakteriellen Konjugation

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13,730 Views

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10:41 min

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January 04, 2017

DOI:

10.3791/54854-v

DOI:

10.3791/54854-v

•

10:41 min

January 04, 2017

•

4 Views

Fettah Erdogan¹, Cristina Lento¹, Ayat Yaseen¹, Roksana Nowroozi-Dayeni¹, Sasha Kheyson¹, Gerald F. Audette^1,2

¹Department of Chemistry,York University, ²The Centre for Research on Biomolecular Interactions,York University

Transkript

Summary

Here, we present a protocol to knockout a gene of interest involved in plasmid conjugation and subsequently analyze the impact of its absence using mating assays. The function of the gene is further explored to a specific region of its sequence using deletion or point mutations.