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Journal / Immunology and Infection / P. aeruginosa Inficeret 3D Co-Kultur af bronkial…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
P. aeruginosa Inficeret 3D Co-Kultur af bronkial epitelceller og makrofager på Air-Liquid Interface for præklinisk evaluering af anti-infektiøs
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P. aeruginosa Inficeret 3D Co-Kultur af bronkial epitelceller og makrofager på Air-Liquid Interface for præklinisk evaluering af anti-infektiøs

Article DOI: 10.3791/61069-v • 10:26 min • June 15th, 2020
June 15th, 2020 •
*1,2, *1,2, 3, 4, 4, 3, 1, 1, 1,2
1Department of Drug Delivery, Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), 2Department of Pharmacy, Saarland University, 3Department of Vaccinology and Applied Microbiology, Helmholtz Centre for Infection Research, 4Department of Internal Medicine V - Pulmonology, Allergology, Respiratory Intensive Care Medicine, Saarland University Hospital
* These authors contributed equally

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Vi beskriver en protokol for en tre-dimensionel co-kultur model af inficerede luftveje, ved hjælp af CFBE41o- celler, THP-1 makrofager, og Pseudomonas aeruginosa, etableret på luft-væske interface. Denne model giver en ny platform til samtidig at teste antibiotika effekt, epitelbarriere funktion, og inflammatoriske markører.

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