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Journal / Immunology and Infection / P. aeruginosa Co-cultura 3D infetta di cellule…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
P. aeruginosa Co-cultura 3D infetta di cellule epiteliali bronchiali e macrofagi all'interfaccia aria-liquido per la valutazione preclinica degli anti-infettivi
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P. aeruginosa Co-cultura 3D infetta di cellule epiteliali bronchiali e macrofagi all'interfaccia aria-liquido per la valutazione preclinica degli anti-infettivi

Article DOI: 10.3791/61069-v • 10:26 min • June 15th, 2020
June 15th, 2020 •
*1,2, *1,2, 3, 4, 4, 3, 1, 1, 1,2
1Department of Drug Delivery, Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), 2Department of Pharmacy, Saarland University, 3Department of Vaccinology and Applied Microbiology, Helmholtz Centre for Infection Research, 4Department of Internal Medicine V - Pulmonology, Allergology, Respiratory Intensive Care Medicine, Saarland University Hospital
* These authors contributed equally

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Descriviamo un protocollo per un modello di co-coltura tridimensionale di vie aeree infette, utilizzando CFBE41o- cellule, macrofagi THP-1 e Pseudomonas aeruginosa, stabilito presso l'interfaccia aria-liquido. Questo modello fornisce una nuova piattaforma per testare simultaneamente l'efficacia antibiotica, la funzione della barriera epiteliale e i marcatori infiammatori.

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Questo mese in JoVE Numero 160 Biofilm antibiotici infezioni polmonari fibrosi cistica infiammazione interleukins TEER alternative ai test sugli animali
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