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Journal / Biochemistry / 单分子鱼的转录活性等位基因单细胞分析…
JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
单分子鱼的转录活性等位基因单细胞分析
A Robust Single-Particle Cryo-Electron Microscopy (cryo-EM) Processing Workflow with cryoSPARC, RELION, and Scipion
Journal (Biochemistry)
A Robust Single-Particle Cryo-Electron Microscopy (cryo-EM) Processing Workflow with cryoSPARC, RELION, and Scipion
Fast Grid Preparation for Time-Resolved Cryo-Electron Microscopy
Journal (Biochemistry)
Fast Grid Preparation for Time-Resolved Cryo-Electron Microscopy
 
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单分子鱼的转录活性等位基因单细胞分析

Article DOI: 10.3791/61680-v • 06:26 min • September 20th, 2020
September 20th, 2020 •
1,2, 1,3, 1,2, 1,2, 1,2,3,4
1GCC Center for Advanced Microscopy and Image Informatics, 2Department of Molecular and Cellular Biology, Baylor College of Medicine, 3Center for Translational Cancer Research, Institute of Biosciences and Technology, Texas A&M University, 4Department of Pharmacology and Chemical Biology, Baylor College of Medicine

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Summary
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单分子RNA荧光就地杂交（smFISH）是一种在单细胞水平上准确量化特定RNA水平和定位的方法。在这里，我们报告我们已验证的实验室协议，用于湿板凳处理、成像和图像分析，用于特定RNA的单细胞量化。

Tags

生物化学，第163期，单分子鱼，成像，图像分析，基因转录
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