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Journal / Biology / Abbildung replikativer Domänen in…
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JoVE Journal Biology
Abbildung replikativer Domänen in ultrastrukturell konserviertem Chromatin mittels Elektronentomographie
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Abbildung replikativer Domänen in ultrastrukturell konserviertem Chromatin mittels Elektronentomographie

Article DOI: 10.3791/62803-v • 14:56 min • May 20th, 2022
May 20th, 2022 •
*1,2, *1,2, *1,3, 1,2, 1, 1, 1, 2, 4, 1,2,5
1Belozersky Institute of Physico-chemical Biology, Lomonosov Moscow State University, 2Faculty of Biology, Lomonosov Moscow State University, 3Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, 4National Research Center, Kurchatov Institute, 5V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology
* These authors contributed equally

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Dieses Protokoll stellt eine Technik zur hochauflösenden Kartierung von Replikationsstellen in strukturell konserviertem Chromatin in situ vor, die eine Kombination aus voreingebetteter EdU-Streptatvid-Nanogold-Markierung und ChromEMT verwendet.

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Biologie Ausgabe 183
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