Korrelieren von Gen-spezifischen DNA-Methylierungsänderungen mit Expression und Transkriptionsaktivität Astrozytäre<em> KCNJ10</em> (Kir4.1)
7,970 Views
•
11:19 min
•
September 26, 2015
DOI:
Summary
Automatically generated
DNA methylation is capable of maintaining stable levels of gene expression as well as allowing for dynamic changes in gene expression in response to a variety of stimuli. We detail techniques that allow the study of gene-specific changes in DNA methylation and the effect of these changes on gene expression.
Verwandte Videos
09:42
Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
Verwandte Videos
9685 Views
13:47
Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models
Verwandte Videos
9545 Views
13:11
Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain
Verwandte Videos
18782 Views
07:50
Immunohistochemical Detection of 5-Methylcytosine and 5-Hydroxymethylcytosine in Developing and Postmitotic Mouse Retina
Verwandte Videos
8935 Views
12:47
Inducing Plasticity of Astrocytic Receptors by Manipulation of Neuronal Firing Rates
Verwandte Videos
14211 Views
12:19
Imaging Intracellular Ca2+ Signals in Striatal Astrocytes from Adult Mice Using Genetically-encoded Calcium Indicators
Verwandte Videos
14834 Views
08:37
Using Fluorescence Activated Cell Sorting to Examine Cell-Type-Specific Gene Expression in Rat Brain Tissue
Verwandte Videos
16891 Views
10:09
Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark
Verwandte Videos
7473 Views
06:47
In Utero Electroporation of Multiaddressable Genome-Integrating Color (MAGIC) Markers to Individualize Cortical Mouse Astrocytes
Verwandte Videos
5502 Views
10:53
Analysis of Astrocyte Territory Volume and Tiling in Thick Free-Floating Tissue Sections
Verwandte Videos
3436 Views
Read Article
Cite this Article
Nwaobi, S. E., Olsen, M. L. Correlating Gene-specific DNA Methylation Changes with Expression and Transcriptional Activity of Astrocytic KCNJ10 (Kir4.1). J. Vis. Exp. (103), e52406, doi:10.3791/52406 (2015).
Copy