Waiting
Login-Verarbeitung ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / Påvisning af postreplikation af huller…
JoVE Journal
Genetics

Ein Abonnement für JoVE ist erforderlich, um diesen Inhalt ansehen zu können. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.

JoVE Journal Genetics
Påvisning af postreplikation af huller akkumulering og reparation i humane celler ved hjælp af DNA-fiberassayet
Analysis of the Ambient Particulate Matter-induced Chromosomal Aberrations Using an In Vitro System
Journal (Genetics)
Analysis of the Ambient Particulate Matter-induced Chromosomal Aberrations Using an In Vitro System
LINE-1 Methylation Analysis in Mesenchymal Stem Cells Treated with Osteosarcoma-Derived Extracellular Vesicles
Journal (Genetics)
LINE-1 Methylation Analysis in Mesenchymal Stem Cells Treated with Osteosarcoma-Derived Extracellular Vesicles
 
Click here for the English version

Påvisning af postreplikation af huller akkumulering og reparation i humane celler ved hjælp af DNA-fiberassayet

Article DOI: 10.3791/63448-v • 10:32 min • February 3rd, 2022
February 3rd, 2022 •
*1, *2, 2, 1
1Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, 2Division of Oncology, Department of Internal Medicine, Washington University in St. Louis
* These authors contributed equally

Kapitel

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Her beskriver vi to modifikationer af DNA-fiberassayet for at undersøge enkeltstrengede DNA-huller i replikerende DNA efter induktion af læsioner. S1-fiberassayet muliggør detektion af postreplikative huller ved hjælp af den ssDNA-specifikke S1-endonuklease, mens gap-filling-analysen muliggør visualisering og kvantificering af gap-reparation.

Tags

Genetik udgave 180 postreplikativt enkeltstrenget DNA-hul mellemrumsfyldning reparation efter replikation DNA-replikation DNA-fiberassay S1-nuklease repriming
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter