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Journal / Immunology and Infection / マウス小腸上皮オルガノイドと先天性リンパ球細胞との共培養…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
マウス小腸上皮オルガノイドと先天性リンパ球細胞との共培養
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マウス小腸上皮オルガノイドと先天性リンパ球細胞との共培養

Article DOI: 10.3791/63554-v • 08:22 min • March 23rd, 2022
March 23rd, 2022 •
*1,2, *1,2,3, 1, 1
1Centre for Host-Microbiome Interactions, King’s College London, 2Wellcome Trust Cell Therapies and Regenerative Medicine Ph.D. Programme, King’s College London, 3Present address: Wellcome Trust/Cancer Research UK Gurdon Institute, Cambridge University
* These authors contributed equally

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Summary
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このプロトコルは、マウス小腸オルガノイドの樹立、マウス小腸固有層からの1型先天性リンパ系細胞の単離、および腸上皮細胞と1型先天性リンパ系細胞との間の双方向相互作用を研究するための両方の細胞型間の3次元(3D)共培養を確立するための詳細な指示を提供する。

Tags

免疫学と感染、第181号、
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