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Immunology and Infection

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Co-kultur av murine tynntarmen epitelial organoider med medfødte lymfoide celler
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Co-kultur av murine tynntarmen epitelial organoider med medfødte lymfoide celler

Article DOI: 10.3791/63554-v • 08:22 min • March 23rd, 2022
March 23rd, 2022 •
*1,2, *1,2,3, 1, 1
1Centre for Host-Microbiome Interactions, King’s College London, 2Wellcome Trust Cell Therapies and Regenerative Medicine Ph.D. Programme, King’s College London, 3Present address: Wellcome Trust/Cancer Research UK Gurdon Institute, Cambridge University
* These authors contributed equally

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Denne protokollen gir detaljerte instruksjoner for å etablere murine tynntarmen organoider, isolere type-1 medfødte lymfoidceller fra murine tynntarmen lamina propria, og etablere 3-dimensjonale (3D) kokulturer mellom begge celletyper for å studere toveis interaksjoner mellom intestinale epitelceller og type-1 medfødte lymfoidceller.

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Immunologi og infeksjon utgave 181
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