Waiting
Login-Verarbeitung ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / E3泛素连接酶在哺乳动物细胞裂解物中底物泛素化的评价…
JoVE Journal
Biochemistry

Ein Abonnement für JoVE ist erforderlich, um diesen Inhalt ansehen zu können. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.

JoVE Journal Biochemistry
E3泛素连接酶在哺乳动物细胞裂解物中底物泛素化的评价
RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs
Journal (Biochemistry)
RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs
High-Throughput Protein Crystallization via Microdialysis
Journal (Biochemistry)
High-Throughput Protein Crystallization via Microdialysis
 
Click here for the English version

E3泛素连接酶在哺乳动物细胞裂解物中底物泛素化的评价

Article DOI: 10.3791/63561-v • 09:47 min • May 10th, 2022
May 10th, 2022 •
*1, *2, 1, 1,2
1Department of Genetic and Evolution, Federal University of São Carlos, 2Department of Biochemistry and Immunology, Faculty of Medicine of Ribeirão Preto, University of São Paulo
* These authors contributed equally

Kapitel

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

我们为哺乳动物细胞中特定底物和E3泛素连接酶的泛素化测定提供了详细的方案。HEK293T细胞系用于蛋白质过表达，通过免疫沉淀从细胞裂解物中纯化多泛型底物，并在SDS-PAGE中分离。免疫印迹用于可视化这种翻译后修饰。

Tags

生物化学，第183期，
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter