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Flowcytometrisk analyse af biomarkører til påvisning af funktionelle defekter i menneskelig sæd
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Flowcytometrisk analyse af biomarkører til påvisning af funktionelle defekter i menneskelig sæd

Article DOI: 10.3791/63790-v • 08:48 min • April 21st, 2022
April 21st, 2022 •
1, 1, 1, 1, 2, 1, 1, 1, 1, 1
1Institute of Toxicology, College of Preventive Medicine, Army Medical University (Third Military Medical University), 2Department of Chemical Defense Medicine, College of Military Preventive Medicine, Army Medical University (Third Military Medical University)

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Denne protokol giver en praktisk løsning, der muliggør måling af apoptose, mitokondriemembranpotentiale og DNA-skader i menneskelig sæd med et enkelt cytometer.

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Flowcytometri biomarkører sædfunktionsdefekter konventionelle sædparametre idiopatisk infertilitet sædapoptose mitokondriemembranpotentiale (MMP) DNA-skader FCM-teknikker Annexin V-fluoresceinisothiocyanat (FITC)/propidiumiodid (PI) farvning 5,5& 8242;,6,6& 8242;-tetrachlor-1,1& 8242;,3,3& 8242;-tetraethyl-benzimidazolylcarbocyaniniodid (JC-1) sonde sædkromatinstrukturassay (SCSA) acridinorange (AO) farvning
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