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Journal / Immunology and Infection / Scheiding van immuuncelsubpopulaties in perifere…
JoVE Journal
Immunology and Infection

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Scheiding van immuuncelsubpopulaties in perifere bloedmonsters van kinderen met infectieuze mononucleosis

Article DOI: 10.3791/64212-v • 08:44 min • September 7th, 2022
September 7th, 2022 •
1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2
1Beijing Key Laboratory of Pediatric Respiratory Infectious Diseases, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, Laboratory of Infection and Virology, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, 2Research Unit of Critical Infection in Children, 2019RU016, Chinese Academy of Medical Sciences

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Summary
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We beschrijven een methode die immunomagnetische kralen en fluorescentie-geactiveerde celsortering combineert om gedefinieerde immuuncelsubpopulaties van perifere mononucleaire bloedcellen (monocyten, CD4 + T-cellen, CD8 + T-cellen, B-cellen en natural killer-cellen) te isoleren en te analyseren. Met behulp van deze methode kunnen magnetische en fluorescerend gelabelde cellen worden gezuiverd en geanalyseerd.

Tags

Immunologie en infectie Nummer 187 Epstein-Barr-virus (EBV) infectieuze mononucleosis (IM) immunomagnetische kraalsortering fluorescente geactiveerde celsortering (FACS) immuuncelsubpopulaties
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